Methods

PROTOCOL FOR DIAGNOSIS
  • Persistent micro and/or recurrent macroscopic hematuria
  • Febrile upper respiratory tract infections or other infections
  • Glomerular mesangial IgA deposits
  • Absence of systemic disease or liver disease


GUIDELINES FOR THE ENROLMENT TO THE STUDY

STEP.1
     To draw the pedigree according to genetic symbols reported below:

STEP.2
     IgA nephropathy patient relatives of at least 3 generations should be checked for urinalysis and their blood samples should be collected, after informed consent, in the following way:

3 EDTA-blood samples (DNA isolation and plasma collection)
1 Blood sample without anticoagulant (serum collection)

for a total volume of 30 ml from adults and 15 ml from children.
The collection of blood samples from IgAN patient, his/her parent (trios) and from all the first degree relatives is very important for genetic studies.

STEP.3
     Whenever urine abnormalities occurs in relatives, it should be documented 3 times (1 time /month) and after that Addis count and renal ultrasound scan should be carried out.

STEP.4
     All the personal and clinical data in an appropriate data sheet must be collected and a complete adjournment of IgAN Consortium database must be done.